As a Regenerative Medicine Specialist with experience in the isolation and deployment of stromal vascular fraction (SVF) containing adipocyte-derived stem cells (ADSCs) for the treatment of degenerative orthopedic conditions since 2011, I have been asked to share my thoughts about the LUNA-STEM that I use in my practice.
During the initial years, I used a manual microscopy method using trypan blue staining to perform cell counts and viability assessments of isolated SVF specimens. During the past year, I purchased a LUNA-STEM that uses AO/PI staining. The LUNA-STEM has increased my time efficiency and accuracy in determining cell counts for nucleated and non-nucleated cells within the SVF specimen, as well as cell viability assessments. The interactive touch screen on the LUNA-STEM is easy to use.
Manual image focusing on an SVF specimen placed on a counting slide, referred to as a PhotonSlide, is very straightforward. Data reports and captured images can quickly be saved as a PDF file on an inserted USB thumb drive. For chart documentation, the LUNA Printer is great for an immediate printout of counting results.
I can honestly say that I will not be going back to my old trypan blue staining method. The LUNA-STEM universally does a better job at cell counting and viability than I can do.