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在使用干细胞或脂肪基质血管组分(SVF)细胞等组织来源细胞时，了解细胞浓度和活力对保证和控制下游手术质量至关重要。组织来源的样本经常被红血球和非细胞碎片污染，使细胞计数成为一个耗时、受主观影响且容易出错的过程。LUNA-STEM™配备了双重荧光和明场光学，以区分活、死有核细胞和无核细胞。样品用核酸染料染色，以确定单核细胞的浓度和性。将无核细胞与碎片区分开并计数。在短短的30秒内，LUNA-STEM™可报告总细胞浓度、有核细胞浓度、无核细胞浓度、细胞活力和平均细胞大小。用绿色圆圈标记活细胞和红色圆圈标记死细胞的高分辨率图像可以直观地确认数据的准确性。
Consistent and accurate day after day!The LUNA-STEM Counter is very quick, effective, and accurate. We use the LUNA-STEM Counter on a daily basis and have run into no troubles for 2 years now. In addition we are a consumer of the LUNA-STEM Slides and AOPI Fluorescent dye, both of which are very inexpensive and extremely obtainable - with help from outstanding customer service. Definitely worth the money.
Best cell counting. Reliable and easy to use.We use it to count mononucleated cells. Very useful and easy to use. Perfect for our daily use.
Hospital de la Santa Creu i Sant Pau
Methods and strategies for procurement, isolation, characterization, and assessment of senescence of human mesenchymal stem cells from adipose tissue.
2019. Gaur M, Dobke M, Lunyak VV. Methods in Molecular Biology.
Serial injections of cryopreserved fat at −196°c for tissue rejuvenation, scar treatment, and volume augmentation.
2018. Ohashi M, Chiba A, Nakai H, Fukuda E, Higuchi T. Plastic and Reconstructive Surgery-Global Open, 6(5): e1742.
Comparison of two automated cell counters for enumeration and viability of mesenchymal stem cells for clinical cellular therapy trials
2018. Radel DJ, Madde P, Dietz A. Cytotherapy, 20(5): S69–S70.
Therapeutic potential of adipose-derived therapeutic factor concentrate for treating critical limb ischemia.
2016. Procházka V, Jurčíková J, Laššák O, Vítková K, Pavliska L, Porubová L, Buszman PP, Krauze A, Fernandez C, Jalůvka F, Špačková I, Lochman I, Jana D, Merfeld-Clauss S, March KL, Traktuev DO, Johnstone BH. Cell transplantation. 25(9): 1623-1633.
The safety and efficacy of cell-assisted fat grafting to traditional fat grafting in the anterior mid-face: an indirect assessment by 3D imaging.
2015. Sasaki GH. Aesthetic Plastic Surgery, 39(6):833-46.
| Cat # | Product | Qty |
|---|---|---|
| L30001 | LUNA-STEM™ Automated Fluorescence Cell Counter for Stem Cells & SVF | 1 unit |
| L12005 | PhotonSlide™, 50 Slides | 1 box |
| L12006 | PhotonSlide™, 500 Slides | 10 boxes |
| L12007 | PhotonSlide™, 1000 Slides | 20 boxes |
| L12011 | LUNA™ Reusable Slide | 1 unit |
| L12012 | LUNA™ Reusable Slides | 2 units |
| L12014 | LUNA™ Reusable Slide Coverslips | 10 units |
| F23001 | Acridine Orange/Propidium Iodide Stain | 2 x 0.5 mL |
| F23002 | Acridine Orange Stain | 2 x 0.5 mL |
| F23003 | Propidium Iodide Stain | 2 x 0.5 mL |
| F23212 | Cell Dilution Buffer | 5 x 20 mL |
| F23102 | LUNA™ Fluorescence Calibration Beads | 1 x 0.5 mL |
| L34003 | LUNA-STEM™ IQ/OQ Protocol | 1 copy |
| P10001 | LUNA™ Printer I | 1 unit |
| P12001 | LUNA™ Printer I Paper (Thermal, 275 prints/roll, 6rolls) | 3 x 2 rolls |
| Sample Volume | 10 μl |
| Cell Counting Time | 30-60 sec (depending on sample conditions) |
| Cell Concentration Range | 5×104 – 1×107 cells/mL (optimal range) |
| Cell Size Range | Detectable Range: 1 – 90 μm Optimal Range: 5 – 60 μm |
| Excitation wavelength | 470 ± 25 nm |
| Emission wavelength | 530 ± 25 nm, 600 nm (LP) |
| Light Source | LED |
| Image Resolution | 5 MP |
| LCD Display | 7 inches (800 x 480 pixels) |
| Dimensions (W×D×H) | 22 × 21 × 9 cm (8.6 × 8.3 × 3.5 in) |
| Weight | 1.8 kg (4 lb) *without the AC adapter |
