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尽管细菌比真核生物更小、更简单，但它们是一类令人难以置信的多样化的生物体，具有多种形状、大小和排列方式。菌落计数是一个高度可变和不可靠的，因为它只是对存在的活细胞的一个估计。即使是昂贵的流式细胞仪和激光扫描细胞仪，也会将每个颗粒，不论是单个还是成团的记录为单个事件。QUANTOM Tx™微生物细胞计数仪是一种基于图像的自动细胞计数装置，可以在短短几分钟内检测单个细菌细胞。全新的的针对细菌优化的细胞检测软件可以在最紧密的细菌团块中计数单个细菌细胞。
Great machine with simple precision that's reliable.
Overall, QUANTOM Tx is user-friendly, simple, quick, but accurate and accountable. The whole measurement for total and viable counts can be optimized to as short as 30 min for the entire process. The results are reliable and accountable. I'd recommend this for all cell biology work. Compared with traditional cell plating which requires overnight incubation for viable results or total cell count by using the Neubauer method. With QUANTOM Tx, we can have reliable results and reduce the total process time.
Overall, we are very satisfied with the platform. It is a mainstay of our workflow.
We use the Logos QUANTOM Tx (QTx) to enumerate various species of bacteria grown in liquid media. Prior to purchasing the QTx, enumeration was done via the spread-plate method. The rapid quantitation afforded by the QTx allows us to have a result in as little as 30 minutes, compared to 3 days for the spread-plate method. We have also found the QTx useful for determining the proportion of live/dead cells within a sample. The simplicity and ease of use of the QTx allows for rapid training of new operators and excellent repeatability. In addition, Logos' after-sales service has been exceptional.
Servatus Ltd
Potassium Lactate as a Strategy for Sodium Content Reduction without Compromising Salt-Associated Antimicrobial Activity in Salami.
2021. Muchaamba F, Stoffers H, Blase R, Ah UV, Tasara T. Foods 10(1):114.
Early Cytokine Induction Upon Pseudomonas aeruginosa Infection in Murine Precision Cut Lung Slices Depends on Sensing of Bacterial Viability.
2020. Kolbe U, Yi B, Poth T, Saunders A, Boutin S, Dalpke AH. Frontiers in Immunology 11:598636.
Low-Carbohydrate Tolerant LAB Strains Identified from Rumen Fluid: Investigation of Probiotic Activity and Legume Silage Fermentation.
2020. Kuppusamy P, Kim D, Soundharrajan I, Park HS, Jung JS, Yang SH, Choi KC. Microorganisms 8(7):E1044.
Potential clinical application of an automated fluorescent microbial cell counter in the detection of urinary tract infection.
2020. Phillips LE, Verma S, Surapaneni BK, Dutta SK. Journal of Clinical Laboratory Analysis 4:e23334.
Probiotic Characteristics and Antifungal Activity of Lactobacillus plantarum and Its Impact on Fermentation of Italian Ryegrass at Low Moisture.
2020. Muthusamy K, Soundharrajan I, Sriseharam S, Kim DH, Kuppusamy P, Lee KD, Choi KC. Applied Sciences 2020, DOI:10.3390/app10010417.
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| Cat # | Product | Qty |
|---|---|---|
| Q10001 | QUANTOM Tx™ Microbial Cell Counter | 1 unit |
| Q10002 | QUANTOM™ Centrifuge | 1 unit |
| Q12001 | QUANTOM™ M50 Cell Counting Slides, 50 Slides | 1 box |
| Q12002 | QUANTOM™ M50 Cell Counting Slides, 500 Slides | 10 boxes |
| Q13501 | QUANTOM™ Total Cell Staining Kit - QUANTOM™ Total Cell Staining Dye (1 x 0.25 mL) - QUANTOM™ Total Cell Staining Enhancer (1 x 0.5 mL) - QUANTOM™ Cell Loading Buffer I (2 x 1 mL) | 1 kit |
| Q13502 | QUANTOM™ Viable Cell Staining Kit - QUANTOM™ Viable Cell Staining Dye (2 x 50 µg) - Dimethyl Sulfoxide (2 x 1 mL) - QUANTOM™ Viable Cell Dilution Buffer (1 x 20 mL) - QUANTOM™ Cell Loading Buffer I (2 x 1 mL) | 1 kit |
| Q13102 | QUANTOM™ Calibration Beads | 1 x 0.5 mL |
| Physical Characteristics | Instrument type Benchtop bacterial cell counter Touchscreen 10″ capacitive touchscreen, 1280 x 800 pixels Dimensions 43.3 × 31.0 × 22.5 cm (17.0 x 12.2 x 8.8 in) Weight 10.8 kg (23.9 lb) |
| Technical Specifications | Cell detection method Automated fluorescence microscopy Processing time ~ 1 minute (to capture and analyze 20 images) Sample concentration range 2 x 105 to 1 x 109 cells/mL (optimal: 1 x 106 to 5 x 108 cells/mL) Cell size range 0.3-50 µm Sample volume Loading volume: 5-6 µL, measuring volume: 0.17 µL (20 images) |
| Physical Characteristics | Instrument type Benchtop centrifuge Dimensions 21 x 21 x 22 cm (8.3 x 8.3 x 8.7 in) Weight 5.64 kg (12.4 lb) |
| Technical Specifications | Maximum capacity Up to 8 QUANTOM™ M50 Cell Counting Slides Maximum RPM 4,000 RPM Safety features Safety lid lock, lid drop protection, automatic door release Electrical requirements 110 V, 60 Hz, 1 A or 220-240 V, 50/60 Hz, 0.5 A |
| Physical Characteristics | Material Poly(methyl methacrylate) (PMMA) Dimensions 25 x 75 x 1.65 mm Chamber volume 5-6 µL |
